Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors    Moretti S, Cavanna D, Lambertini F, Catellani D, Sammarco G, Barola C, Paoletti F, Saluti G, Galarini R, Suman M

Abstract   A multi-group screening method to detect residues of veterinary drugs in meat and environmental contaminants in wheat flour has been developed using liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS). The procedure was tested for over 300 representative compounds (173 veterinary drugs, 122 pesticides and 9 mycotoxins) analysing in parallel negative and positive (spiked) samples according to European validation rules. The Screening Target Concentrations (STCs) were chosen conservatively with respect to the method purposes. Interpretation of results was based on retention time, mass accuracy of precursor and MS² spectral library. Evaluating the percentage of false negative results, 280 out of the 304 analytes were detectable at the STCs (false compliant rate ≤ 5%). In wheat flours, incurred levels of mycotoxins, deoxynivalenol and 3-acetyldeoxynivalenol, higher than STCs, were frequently found, whereas in meat, the most detected veterinary drugs were antibiotics generally at negligible concentrations (<10 μg kg-¹ ). Finally, seven test materials from proficiency test schemes were successfully tested.

Publish Date     2020

Volume    55 (11)

ISSN        1096-9888

DOI          doi.org/10.1002/jms.4618

URL          https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jms.4618

Journal    Journal of Mass Spectrometry

Pages       art. n. 4618

PMID       32757493