Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors    Giusepponi D, Galarini R, Barola C, Torquato P, Bartolini D, Moretti S, Saluti G, Gioiello A, Libetta C, Galli F

Abstract    The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM^® 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown.

Publish Date   2019

Volume     144

ISSN     1873-4596

DOI     doi.org/10.1016/j.freeradbiomed.2019.04.017

URL    https://www.sciencedirect.com/science/article/pii/S0891584918324250?via%3Dihub

Journal    Free Radical Biology and Medicine

Pages  134-143

PMID   31009660