Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors   Ianni F, Volpi C, Moretti S, Blasi F, Mondanelli G, Varfaj I, Galarini R, Sardella R, Di Renzo G C, Cossignani L

Abstract   In this research, the phenolic extract from Moraiolo extra-virgin olive oil (EVOO) was thoroughly characterized. A reversed-phase HPLC method with a photodiode detector allowed to measure a total phenol content higher than 500 mg/kg EVOO, with elevated amounts of oleocanthal, oleacein, and oleouropein aglycone (131.2, 213.5, and 158.4 mg/kg EVOO, respectively). Appreciable amounts of (+)-pinoresinol and (+)- 1-acetoxy-pinoresinol, 3.2 and 12.5 mg/kg EVOO respectively, were measured. High-resolution mass spectrometry with Orbitrap mass analyzer technology was used to confirm the identity of the analytes. Afterwards, the extract was tested, for the first time, for its activity on Indoleamine-2,3-Dioxygenase (IDO1). This enzyme appears as a promising target for the modulation of the neuroinflammatory-oxidative processes relying on the pathogenesis of several neurodegenerative diseases. The extract showed an inhibitory effect on the catalytic activity of both human and murine IDO1, with a good safety at the concentrations of 15 and 30 µg/mL.

Publish Date   2022

Volume   213

ISSN    1873-264X

DOI   doi.org/10.1016/j.jpba.2022.114688

URL   https://www.sciencedirect.com/science/article/pii/S0731708522001091?via%3Dihub

Journal    Journal of Pharmaceutical and Biomedical Analysis

Pages   Article no. 114688

PMID   35220200