Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors   Giusepponi D, Paoletti F, Barola C, Moretti S, Saluti G, Ianni F, Sardella R, Galarini R

Abstract   A multiclass method has been developed to screen and confirm a wide range of anti-microbial residues in muscle and milk, and validated using liquid-chromatography coupled to (low-resolution, LR) tandem mass spectrometry (LC-QqQ). Over sixty antibiotics, belonging to ten distinct families, were included in the method scope. The development process was rapidly concluded as a result of two previously implemented methods. This consisted of identical sample treatments, followed by liquid chromatography, and coupled with high-resolution (HR) mass spectrometry (LC-Q-Orbitrap). The validation study was performed in the range between 10-1500 μg·kg^-1 for muscles and 2-333 μg·kg^-1 for milk. The main performance characteristics were estimated and, then, compared to those previously obtained with HR technique. The validity of the method transfer was ascertained also through inter-laboratory studies.

Publish Date    2019

Volume   24 (16)

ISSN  1420-3049

DOI   doi.org/10.3390/molecules24162935

URL   https://www.mdpi.com/1420-3049/24/16/2935

Journal    Molecules

Pages   E2935

PMID  31412663