Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors  Sgariglia E, Aconiti Mandolini N, Napoleoni M, Medici L, Fraticelli R, Conquista M, Gianfelici P, Staffolani M, Fisichella S, Capuccella M, Sargenti M, Perugini G

Abstract  Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are the main weapon to reduce incidence and mortality associated to it. However, indiscriminate use of antibiotics may lead to therapy failure and economic losses for the breeder. The aims of this study were to, determine the antibiotic resistance of Escherichia coli isolates, evaluate the correlation between E. coli isolation and systems of breeding included in this study, and identify the avian pathogenic E.coli (APEC) amongst the E. coli strains isolated. A total of 51 E. coli strains were subjected to antimicrobial susceptibility test and they were screened for the presence of virulence genes through PCR. Resistance was most frequently detected against ampicillin and nalidixic acid meanwhile E. coli isolates showed less resistance to the cephalosporins. Overall, 40% of the isolates showed resistance to at least three or more antimicrobials and 16/51 isolates were defined APEC strains. The virulence genes iucD, cvi/cva, irp2 and iss were detected from all 16 APEC strains. The virulence genes tsh, vat, papC, and astA were detected from 11, 7, 5 and 3 APEC strains, respectively. Results demonstrated the importance of studies on APEC and antibiotic resistance genes in Italy, and it was shown that the systems of breeding might influence the antibiotic resistanc.

Publish Date  2019

Volume  55 (1)

ISSN  1828-1427

DOI  doi.org/10.12834/VetIt.1617.8701.1

URL  https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1617

Journal  Veterinaria italiana

Pages  26-33

PMID    30951179