TRADITIONAL PCR AND REAL-TIME PCR ARE COMPLEMENTARY TOOLS TO IMPROVE THE DIAGNOSIS OF ENZOOTIC BOVINE LEUKOSIS
Authors Bazzucchi M, Casciari C, Iscaro C, Giammarioli M, Farneti S, Feliziani F
Abstract Traditional PCR and Real-Time PCR are Complementary Tools to Improve the Diagnosis of Enzootic Bovine Leukosis Background: Enzootic Bovine Leukosis (EBL) is an infectious disease of cattle caused by a deltaretrovirus, namely the bovine leukaemia virus (BLV). The use of serological tests (AGID; ELISA) is prescribed by the OIE under eradication plans for the disease; however, no gold standard for the diagnosis of this disease is available at present. The utility of traditional PCR and real-time PCR methods has been suggested by the Italian Reference Laboratory and evaluated using blood as well as tissue samples. Objective: The aim of this study is explore two in house biomolecular test to improve EBL diagnosis. Methods: We compare an end point PCR, a Real Time PCR each other and with serological tests as AGID and ELISA. Results: The molecular techniques revealed a complete agreement. However, comparison of results obtained by serological investigation and the molecular techniques indicated differences. Despite the lack of sensitivity of these methods, particularly in tissue samples, both the tests were found to be able to detect the BLV provirus in the present study. Conclusion: Although serological tests were confirmed to be the most reliable, practical, and economic for use in BLV surveillance plans, biomolecular tests represent a viable alternative as a support tool for use in research studies, in cases in which serum samples are unavailable, or when serological diagnosis yields inconclusive results.
Publish Date 2018
Volume 7 (5)
ISSN 2325-9590
DOI No
URL No
Journal Journal of Veterinary Science & Medical Diagnosis
Pages No
PMID No