Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ''Togo Rosati''

Authors Paoloni A, Solfrizzo M, Bibi R, Pecorelli I

Abstract The objective of this study was to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Ochratoxin A (OTA) and Ochratoxin α (OTα) in poultry tissues and eggs. The two toxins were extracted by a mixture of acetonitrile/water, purified with a reversed phase C18 solid phase extraction column (SPE) and determined by LC-MS/MS. The LC-MS/MS method performances were evaluated in terms of linearity in solvent and in matrix (ranged from 0.5 to 15.10 µg L^-1 for OTA and from 0.60 to 17.85 µg L^-1 for OTα), limit of detection (LOD), limit of quantitation (LOQ), specificity, accuracy and precision in repeatability conditions. Recovery experiments were performed by spiking poultry liver, kidney, muscle and eggs around 1 µg kg^-1 and 10 µg kg^-1. LODs were 0.27 and 0.26 µg kg^-1 while LOQs were fixed at 1.0 and 1.2 µg kg^-1 for OTA and OTα, respectively. Main recoveries for OTA ranged from 82 to 109% and for OTα ranged from 55 to 89%. The values of within-laboratory relative standard deviation (RSD_r) were equal to or below 20%. Considering the results obtained and that all analytical performance criteria were fulfilled, the new extraction and purification method developed for OTA and OTα determination in animal tissues and eggs was found appropriate for control laboratories and research activities designed to ensure food safety.

Publish Date 2018

Volume 53(5)

ISSN 1532-4109

DOI doi.org/10.1080/03601234.2018.1431455

URL https://www.tandfonline.com/doi/full/10.1080/03601234.2018.1431455

Journal Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes

Pages 327-333

PMID 29393822